325 research outputs found

    The Effect of Galactose on the Expression of Genes Regulated by Rrp6p

    Get PDF
    Gene expression is a multi-faceted phenomenon, governed not only by the sequence of nucleotides, but also by the extent to which a particular gene gets transcribed, how the transcript is processed, and whether or not the transcript ever makes it out of the nucleus. Rrp6p is a 5’-3’ exonuclease that can function independently and as part of the nuclear exosome in Saccharomyces cerevisiae (Portin, 2014). It degrades various types of aberrant RNA species including small nuclear RNAs, small nucleolar RNAs, telomerase RNA, unspliced RNAs, and RNAs that have not been properly packaged for export (Butler & Mitchell, 2010). This exosome mediated degradation is important as the accumulation of unprocessed mRNA transcripts can be harmful to the cell. These experiments sought to quantify changes in RNA levels in rrp6-Δ strains grown in glucose versus those grown in galactose compared to wild type strains grown in each of the carbon sources. The goal was to determine if there was an interplay between the genes regulated by Rrp6p and the genes involved in the switch from glucose metabolism to galactose metabolism. Based on the data, it appears that the absence of Rrp6p increases transcription in glucose while, in galactose, the absence of Rrp6p increases transcription to a significantly lesser degree than in glucose and in some cases decreases transcription

    Sorrows (A Sestina)

    Get PDF

    Open House: Connected Homes and the Curtilage

    Get PDF

    Craftivist Clay: Resistance and Activism in Contemporary Ceramics

    Get PDF
    Abstract Craftivist Clay: Resistance and Activism in Contemporary Ceramics Mary Callahan Baumstark Master of Arts in Contemporary Art, Design, and New Media Histories OCAD University April 2016 Since the late twentieth century, the social engagement of craft emerged as a primary concern for both makers and activists. While “craftivism” was quickly defined by the work of a few, this thesis expands previous considerations of craftivism as both theoretical construct and making method. Considering the emergence of craftivism as method, this paper examines the work of ceramic craftivists, analyzing their practices and produced works within a context of increased political urgency, using ceramics as a microcosmic exploration for craftivism in a given craft media. This thesis will analyze the efficacy of this method in contemporary ceramics, seeking to clarify both the import and impact of craftivism in a world overwhelmed by both activist causes and material goods. This thesis questions and complicates the history of craftivist theory, its role in contemporary ceramic practice, and its efficacy as a whole, exploring the extent to which craft can create social change. Keywords: craftivism, craft, ceramic, maker, activism, method, feminism, clay, activist, objec

    Investigating Metrics Proposed to Prevent the Harvest of Leafy Green Crops Contaminated by Floodwater

    Get PDF
    Pathogens can be transported by water through soil to contaminate distant crops. The California LGMA states that leafy green crops within 30ft of flooded soil should be destroyed due to potential contamination. Previously flooded areas should not be replanted for 60 days. This study investigated the transport of Salmonella enterica and Citrobacter freundii through soil in a model system with a positive slope (uphill). Field trials involving flooding one end of a spinach bed with a negative slope (downhill) with water containing Escherichia coli were also conducted. Soil type, soil moisture content, and slope affected bacterial movement. In field trials, E. coli was quickly transported to the 30ft boundary, and persisted significantly longer in the fall trial than the spring. These data suggest the LGMA metrics need to provide additional parameters to prevent the harvest of leafy green crops potentially contaminated by floodwater

    Predicting risk of hospitalisation: a retrospective population-based analysis in a paediatric population in Emilia-Romagna, Italy.

    Get PDF
    OBJECTIVES: Develop predictive models for a paediatric population that provide information for paediatricians and health authorities to identify children at risk of hospitalisation for conditions that may be impacted through improved patient care. DESIGN: Retrospective healthcare utilisation analysis with multivariable logistic regression models. DATA: Demographic information linked with utilisation of health services in the years 2006-2014 was used to predict risk of hospitalisation or death in 2015 using a longitudinal administrative database of 527 458 children aged 1-13 years residing in the Regione Emilia-Romagna (RER), Italy, in 2014. OUTCOME MEASURES: Models designed to predict risk of hospitalisation or death in 2015 for problems that are potentially avoidable were developed and evaluated using the C-statistic, for calibration to assess performance across levels of predicted risk, and in terms of their sensitivity, specificity and positive predictive value. RESULTS: Of the 527 458 children residing in RER in 2014, 6391 children (1.21%) were hospitalised for selected conditions or died in 2015. 49 486 children (9.4%) of the population were classified in the \u27At Higher Risk\u27 group using a threshold of predicted risk \u3e2.5%. The observed risk of hospitalisation (5%) for the \u27At Higher Risk\u27 group was more than four times higher than the overall population. We observed a C-statistic of 0.78 indicating good model performance. The model was well calibrated across categories of predicted risk. CONCLUSIONS: It is feasible to develop a population-based model using a longitudinal administrative database that identifies the risk of hospitalisation for a paediatric population. The results of this model, along with profiles of children identified as high risk, are being provided to the paediatricians and other healthcare professionals providing care to this population to aid in planning for care management and interventions that may reduce their patients\u27 likelihood of a preventable, high-cost hospitalisation
    • …
    corecore